POLYPEN RP 410 Leaf analyzer for calculating reflectance index formulas

PolyPen RP 410

The PolyPen RP 410 features a complete system for measuring the spectral reflectance of leaves. The device is equipped with an internal light source (380 to 1050 nm xenon incandescent lamp) and with one type of detector (UVIS or NIR, read more under Versions).

The PolyPen RP 410 incorporates commonly used reflectance index formulas (e.g. NDVI, NDGI, PRI, etc.) and allows custom indexes to be programmed. The measured data is instantly displayed in graphs or data sheets on the device's display. It is also stored as a full spectrum in the device's memory for later retrieval or transfer to a PC. The rechargeable lithium-ion battery, splash-proof housing and integrated GPS module make the PolyPen RP 410 an ideal tool for field experiments.

POLYPEN MEASUREMENTS

Absorbance: data calculated using the following formula
A=log(I0/I), where I0 is the reference light intensity and I is the measured light intensity

Transmittance: data calculated using the following formula
T= I/ I0, where I0 is the reference light intensity and I is the measured light intensity

LIST OF CALCULATED VEGETATION INDICES

Normalized difference vegetation index (NDVI)▪
Reference: Rouse et al. (1974) Equation: NDVI = (RNIR - RRED ) / (RNIR + RRED )
Simple ratio index (SR)▪
Reference: Jordan (1969); Rouse et al. (1974) Equation: SR = RNIR / RRED
Modified chlorophyll absorption index reflectance (MCARI1)
Reference: Haboudane et al. (2004) Equation: MCARI1 = 1.2 * [2.5 * (R790- R670) - 1.3 * (R790- R550)]
Optimized soil-adjusted vegetation index (OSAVI)▪
Reference: Rondeaux et al. (1996) Equation: OSAVI = (1 + 0.16) * (R790- R670) / (R790- R670 + 0.16)
Greenness index (G)
Equation: G = R554 / R677
Modified chlorophyll absorbance index (MCARI)
Reference: Daughtry et al. (2000) Equation: MCARI = [(R700- R670) - 0.2 * (R700- R550)] * (R700/ R670)
Transformed CAR index (TCARI)
Reference: Haboudane et al. (2002) Equation: TCARI = 3 * [(R700- R670) - 0.2 * (R700- R550) * (R700/ R670)]
Triangular vegetation index (TVI)
Reference: Broge and Leblanc (2000) Equation: TVI = 0.5 * [120 * (R750- R550) - 200 * (R670- R550)]
Zarco-Tejada and Miller index (ZMI)▪
Reference: Zarco-Tejada et al. (2001) Equation: ZMI = R750 / R710
Simple proportion pigment index (SRPI)
Reference: Peñuelas et al. (1995) Equation: SRPI = R430 / R680
Normalized pheophytinization index (NPQI)
Reference: Barnes et al. (1992) Equation: NPQI = (R415- R435) / (R415+ R435)
Photochemical reflectance index (PRI)
Reference: Gamon et al. (1992) Equation: PRI = (R531- R570) / (R531+ R570)
Normalized pigment chlorophyll index (NPCI)
Reference: Peñuelas et al. (1994) Equation: NPCI = (R680- R430) / (R680+ R430)
Carter Indices
Reference: Carter (1994), Carter et al. (1996) Equation: Ctr1 = R695 / R420; Ctr2▪ = R695 / R760
Specific normalized pigment difference a (PSNDa)▪
Reference: Blackburn (1998) Equation: PSNDa = (R790 - R680) / (R790 + R680)
Structure-insensitive pigment index (SIPI)
Reference: Peñuelas et al. (1995) Equation: SIPI = (R790 - R450) / (R790 - R650)
Gitelson and Merzlyak indices
Reference: Gitelson&Merzlyak (1997) Equation: GM1 = R750/ R550; GM2 ▪= R750/ R700
Anthocyanin reflectance indices (ARI1; ARI2)
Reference: Gitelson et al. (2001) Equation: ARI1 = 1/R550 - 1/R700; ARI2 = R790*(1/R550 - 1/R700)
Carotenoid reflectance indices (CRI1; CRI2)
Reference: Gitelson et al. (2002) Equation: CRI1 = 1/R510 - 1/R550; CRI2 = 1/R510 - 1/R700
Renormalized Differential Vegetation Index (RDVI)
Reference: Roujean & Breon (1995) Equation: RDVI = (R780 - R670)/((R780 + R670)^0.5)

▪ Indices also measured by PolyPen NIR

APPLICATIONS
Plant screening and field studies
Stress response
Pigment composition
Plant water content
Nitrogen status
Grain yield

SOFTWARE
Automatic calculation of all commonly used reflectance indices
Calculation of custom indices (possible on request)
Instant data graphs and data sheets
Export to a PC via USB communication
Data browsing and averaging
Data transfer and display routines to Microsoft Excel
Mapping GPS SpectraPen 1.1 (Windows 7 and above)

Accessories

• Control and Data Unit for Handheld Devices
• Small, portable notebook (type according to current availability on the market).
• Spectralon Reflectance Standard
• For the highest diffuse reflectance values - reflectance value 99 %. Spectrally flat over UV-VIS-NIR spectrum. Optically flat to +/- 1 % over the photopic region of the spectrum.
• Self-Adhesive Rubber Pads
• One set includes 10 solid pads (to be attached to the clip) and 10 pads with an opening (to be attached at the optical head).

Accessories

• Battery replacement and device recalibration * Internal rechargeable battery replacement and device recalibration.
• Control and data unit for handheld devices * Small laptop (type according to current market availability).
• Spectralon reflectance standard * For the highest values ​​of diffuse reflectance. Spectrally flat in the UV-VIS-NIR spectrum. Optically flat up to +/- 1 % in the photopic region of the spectrum.
• Self-adhesive rubber pads for PolyPens * One set includes 10 solid pads (for attaching to the clip) and 10 pads with an opening (for attaching to the optical head).